Tis but a flesh wound

Fluorescence recovery after photobleaching (FRAP) is a technique in fluorescence microscopy used to determine the speed of the diffusion of the molecules in a cellular environment. A small region of a cell is shot with a high-power laser beam leading to the instantaneous absence of fluorescence in that small region (bleach), and the time necessary for the fluorescence to return to the bleached area is used to determine the diffusion coefficient of fluorescently labeled molecules.

The comparison of the lateral dynamics of three fluorescent reporter proteins moving through the plasma membrane of yeast Saccharomyces cerevisiae, static Hip1C-GFP (up), highly mobile Gap1C-GFP (middle) and moderately mobile Lyp1-GFP (down).

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