High throughput microscopy is the important tool in systems biology as it provides thorough, yet relatively fast analysis of behavior of a molecule of interest in different cellular environments of all kingdoms of life.
The example of a large-scale analysis of proteins involved in the maintenance of the plasma membrane integrity. Reporter protein associating with the plasma membrane of wild type cells (up) was expressed in yeast knock-out mutant collection from agar plates (bottom right) with ca. 6000 disrupted gene. Loss of association with the membrane (down) indicates the role of the disrupted gene in the organization of the plasma membrane. In this case the disrupted gene codes for the enzyme essential for the biosynthesis of ergosterol, structural component of the yeast plasma membrane.